Immortalized Human Hair Follicle Dermal Papilla Cells - HPV E6/E7

Human Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. Their survival is regulated by signal transduction pathways such as ERK and Akt. These cells can be used for development and evaluation of hair growth products and identifying cell populations within the hair follicle. In addition, they can be used for in vitro screening of androgen blocking reagents since they have androgen receptors.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene.

BioSafety Level: II

Species: Human

Source Organ: Hair Follicle

Growth Properties: Adherent

Morphology: Multipolar

Population Doubling: N/A

Seeding Density: N/A

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0505.

Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C