Exhibit qualities consistent with primary ovarian granulosa cells; this includes cell retraction in response to protein kinase-A activation, the ability to produce progesterone and estradiol, and the enzyme integral to estradiol production, P450arom
Immortalization Method: Human papilloma virus strain 16 (HPV16) oncogenes E6/E7
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Ovary
Organ Type: Ovary
Growth Properties: Adherent
Morphology: Polygonal
Seeding Density: 10,000 cells/cm²
Donor Age: Donor Info Not Disclosed
Donor Gender: Donor Info Not Disclosed
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 5% Cosmic Calf Serum (Hyclone) + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Copland, J. A., Zlatnik, M. G., Ives, K. L., & Soloff, M. S. (2002). Oxytocin receptor regulation and action in a human granulosa-lutein cell line. Biology of reproduction, 66(5), 1230–1236. https://doi.org/10.1095/biolreprod66.5.1230