Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and hTERT gene
BioSafety Level: II
Species: Human
Source Organ: Gingiva
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No.
TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (
TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (
G255) to a final concentration of 1%. Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C