Immortalized Human Gastric Cells (KMU-CS12)

The Immortalized Human Gastric Cells (KMU-CS12) were derived from isolated gastric cell clones developed from gastric biopsy samples and were spontaneously immortalized in subsequent cultures. The growth of this cell line was found to be significantly faster than its parental gastric cells. This cell line shows cancer cell phenotypes with tumorigenic ability as tested in immunodeficient nude mice. Another unique characteristic of the Immortalized Human Gastric Cells (KMU-CS12) is the high expression of homeobox genes: HOXA4, HOXA5, HOXA7, HOXA9, and HOXA13. This makes the cell line useful in the construction of HOXA genes knockdown models as well as in research focusing on the chromosomal mutations in HOXA gene family. The Immortalized Human Gastric Cells (KMU-CS12) is also valuable in cancer research particularly in the etiology and studying new therapeutic strategy for gastric cancer.

Immortalization Method: Spontaneous immortalization in subsequent culture with activated hTERT

BioSafety Level: II

Species: Human

Source Organ: Stomach

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: Oct-4, Musashi-1, TFF-1, CK8, CK18, EMA

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Keratinocyte-SFM (Gibco-Invitrogen Corporation) supplemented with N-acetyl-Lcysteine (NAC; 2 mmol/l) and L-ascorbic acid 2-phosphate (Asc 2P; 0.2 mmol/l), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1% available at abm.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: 1) Immunocytochemical staining to assess gene expression; 2) Cell proliferation and differentiation assay; 3) Spectral karyotype analysis (SKY)

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C