Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level: II
Species: Human
Source Organ: Oral
Growth Properties: Adherent
Morphology: Other
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow V medium available at
abm, Cat. No.
TM015. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (
TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (
G255) to a final concentration of 1%. Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C