HFK-16E6E7 cells are human keratinocytes immortalized by being transfected with the E6 and E7 open reading frames of human papillomavirus type 16 (HPV16) using retroviruses. HFK-16E6E7 cells can be a useful cell line model as HPVs-transfected human epithelial cells for studiying the HPV pathology and the role of HPVs in theetiology of cervical cancer.
Immortalization Method: Immortalized with retrovirus containing the E6 and E7 open reading frames of HPV16
BioSafety Level: II
Organism: Human
Species: Human
Source Organs: Skin
Organ Type: Skin
Growth Properties: Adherent
Morphology: Fibroblast-like
Markers: neomycin resistance
Donor Age: Not disclosed
Donor Gender: Male
Propagation: The base medium for this cell line is Prigrow X medium available at
abm, Cat. No. TM0723.Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
Quality Control: 1) Analysis of provirus DNA; 2) Immunoprecipitation
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Halbert, C. L., Demers, G. W., & Galloway, D. A. (1991). The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. Journal of Virology, 65(1), 473-478.