Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigenBioSafety Level: IISpecies: HumanSource Organ: Abdominal SkinGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: 24-34 hoursSeeding Density: 10,000-15,000 cells/cm²; Recommended split ratio is 1:2 to 1:3Markers: N/ADonor Age: 39Donor Gender: FemaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Keratinocyte Medium Kit available at abm, Cat. No.TM040. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. Quality Control: Real Time PCR was used to quantify the transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C