Description: Immortalized Human Endometriotic Cell Line (12Z) was generated by infecting cells, obtained from a 37 year-old female undergoing laparoscopy, with SV40 virus. This cell line exhibits expression of markers of endometriotic lesions that are seen in vivo. Immortalized Human Endometriotic Cell Line (12Z) may be used to characterize and identify genes and/or markers related to endometriosis.Immortalization Method: Immortalized with SV40 virusBioSafety Level: IISpecies: HumanSource Organ: UterusGrowth Properties: AdherentMorphology: Fibroblast-likePopulation Doubling: 55 - 65 hoursSeeding Density: 15,000 - 20,000 cells/cm10²Markers: SV40T, cytokeratin, vimentin, N-cadherin, estrogen receptor α and β, progesterone receptor, and aromatase P450Donor Age: 37 years oldDonor Gender: FemaleDonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) Immunofluorescence analysis; 2) Western blot; 3) RT-PCRShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C