Immortalized Human Endometrial Stromal Cells-SV40

Immortalization Method: Serial passaging and infection with Lentivirus carrying SV40 antigen gene

BioSafety Level: II

Species: Human

Source Organ: Reproductive system

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 24-28 hours

Seeding Density: 30,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C