Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying hTERT geneBioSafety Level: IISpecies: HumanSource Organ: LungGrowth Properties: AdherentMorphology: Fibroblast-likePopulation Doubling: 30 - 35 hoursSeeding Density: 10,000 - 20,000 cells/cm10²Markers: N/ADonor Age: Fetal, 3 monthsDonor Gender: FemaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C