Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene.BioSafety Level: IISpecies: HumanSource Organ: SkinGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: N/ASeeding Density: 5,000 - 6,000 cells per cm²Markers: Factor VIII- related antigenDonor Age: 49Donor Gender: MaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify the transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C