Immortalized Human Cranial Perisoteal Cells (Tag58) is the first human cranial periosteal cell line isolated from healthy adult patient biopsy and immortalized by lentiviral transduction with the large SV40 T antigen. Tag58 cells maintain long-term cell proliferation and osteogenic differentiation potential showing stronger mineralization. Tag58 cells express high levels of stem cell surface markers, CD146, and MSCA-1 (mesenchymal stem cell antigen-1), as well as high constitutive mRNA levels of key factors of osteogenesis. Tag58 cell line represents a suitable experimental platform for studying periosteal cell biology, bone formation, and bone regeneration.
Immortalization Method: Immortalized with lentivirus containing large SV40 T antigen
BioSafety Level: II
Species: Human
Source Organ: Bone
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: N/A
Markers: CD44, CD73, CD90, CD105, CD146, CD166, MSCA-1 and low expression of CD34; neomycin resistance (0.25 mg/mL G418)
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Change media every 2-3 days. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
The osteogenic differentiation medium for this cell line is complete culture medium plus the following supplements: 10 mM ß-glycerophosphate disodium salt hydrate (Sigma), 100 μM L-ascorbic acid 2-phosphate (Sigma), and 4 μM dexamethasone (Sigma).
The osteogenic differentiation medium should be always prepared fresh.
Quality Control: 1) Osteogenic differentiation experiments; 2) Western blot; 3) Flow cytometry; 4) Detection of cell mineralization by fluorescent OsteoImage staining; 5) Gene expression analysis
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C