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Immortalized Cells
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Immortalized Primary Cells
>
Immortalized Human Coronary Artery Smooth Muscle Cells
(Click to display a larger image)
Immortalized Human Coronary Artery Smooth Muscle Cells
From Transfected Primary Cells
Catalog number:
ABMT0557
Quantity:
1x10⁶ cells / 1.0 ml
Price inquiry:
info@3hbiomedical.com
Article number:
ABMT0557
Unit:
pcs
Stock status:
In stock
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Description
Immortalization Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 whole gene (
BioSafety Level:
II
Species:
Human
Source Organ:
Coronary Arteries
Growth Properties:
Adherent
Morphology:
Multipolar
Population Doubling:
35-40 hours
Seeding Density:
10,000 - 20,000 cells/cm²; recommended split ratio of 1:2 to 1:4
Markers:
Smooth Muscle Actin
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow II medium available at
abm
, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO
2
): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Real Time PCR was used to quantify the transgene expression.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180°C
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