Immortalized Human Conjuctival Epithelial Cells - SV40

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Eye

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 35-45 hours

Seeding Density: 30,000 - 40,000 cells/cm2

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X.1 series medium available at abm, Cat. No. TM010.

Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C