Immortalized Human Chondrocytes - hTERT

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene

BioSafety Level: II

Species: Human

Source Organ: Normal human articular cartilage

Growth Properties: Adherent

Morphology: Multipolar

Population Doubling: 41-51 hours

Seeding Density: 25,000-30,000 cells/cm²; Recommended split ratio is 1:2 to 1:3

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 1% L-glutamine, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C