Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying hTERT geneBioSafety Level: IISpecies: HumanSource Organ: Normal human articular cartilageGrowth Properties: AdherentMorphology: MultipolarPopulation Doubling: 41-51 hoursSeeding Density: 25,000-30,000 cells/cm²; Recommended split ratio is 1:2 to 1:3Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 1% L-glutamine, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C