Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigenBioSafety Level: IISpecies: HumanSource Organ: Carotid ArteryGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: N/ASeeding Density: 5,000 - 10,000 cells/cm²Markers: Factor VIII-related antigenDonor Age: 26Donor Gender: MaleDonor Ethnicity: OtherPropagation: The base medium for this cell line is Prigrow I medium available at abm, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C