Human Cardiomyocytes are isolated from human heart ventricles. Their specialized high-oxygen-content with a large number of mitochondria contributes to the major role of cardiac muscles in the heart’s rhythmic pumping. Cardiomyocytes are regulated by a complex network of signals. Cardiomyocytes hypertrophy and apoptosis have been associated with the loss of contractile function during heart failure. They are ideal models for study of cytokines and cellular signaling mechanisms that lead to myocyte death, as well as for research on mechanical strain and cell-cell interaction.Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying CDK4, hTERT, and BMI1 genesBioSafety Level: IISpecies: HumanSource Organ: VentricleGrowth Properties: AdherentMorphology: MultipolarPopulation Doubling: 15 - 25 hoursSeeding Density: 10,000 - 20,000 cells/cm²Markers: Puromycin resistance, GATA-4, ACTN2, ACTN3 & MYH2Donor Age: 33 years oldDonor Gender: FemaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow X medium available at abm, Cat. No. TM0539.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Quality Control: N/AShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C