Human Cardiac Fibroblast is an important heart component that provides structural support for cardiomyoctes. They synthesize extracellular matrix, growth factors and cytokines during growth. In addition, they also interact with myocardial responses to injury and pathophysiological conditions such as scar formation at sites of myocardial infarction, cardiac fibrosis and cardiac hypertrophy. These cells are ideal models for study in cardiac matrix response modeled by physiological and pathological stressors. Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigenBioSafety Level: IISpecies: HumanSource Organ: Ventricles (Adult Heart)Growth Properties: AdherentMorphology: MultipolarPopulation Doubling: 48-72 hoursSeeding Density: 3,500-7,000 cells/cm²Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, nonessential amino acids (1 mM), L-glutamine (G275) (2mM), insulin (5µg/mL) (Z101065) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify the SV-40 transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C