Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigenBioSafety Level: IISpecies: HumanSource Organ: LungGrowth Properties: AdherentMorphology: MultipolarPopulation Doubling: 20 - 30 hoursSeeding Density: 20,000 - 30,000 cells/cm²Markers: smooth muscle alpha actinDonor Age: 27Donor Gender: MaleDonor Ethnicity: CaucasianPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, sodium pyruvate to a final concentration of 1 mM, L-glutamine (G275) to a final concentration of (2 mM), nonessential amino acids to a final concentration of 1% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify the transgene expression.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C