Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4
Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying hTERT and CDK4 genes
BioSafety Level: II
Species: Human
Source Organ: Lung
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: Doubling time of approx. 23 hours
Seeding Density: 5,000-7,500 cells per cm²
Markers: Cytokeratin
Donor Age: 55
Donor Gender: Female
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0497. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Quality Control: Real Time PCR was used to quantify hTERT and BMI gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C