Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying hTERT and BMI genesBioSafety Level: IISpecies: HumanSource Organ: LungGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: Doubling time of approx. 23 hoursSeeding Density: 5,000 - 7,500 cells per cm²Markers: CytokeratinDonor Age: 55Donor Gender: FemaleDonor Ethnicity: N/APropagation: Use of Gelatin Coating Solution (0.1%) (TM063) is required for cell adhesion to the culture vessels. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0498. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Quality Control: Real Time PCR was used to quantify hTERT and BMI gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C