Immortalized Human Bone Marrow-derived Stromal Cells - SV40 - YFP

The Immortalized Human Bone Marrow-derived Stromal Cells - SV40 - YFP stably expressed YFP, which makes this cell line a very useful tool in fluorescence microscopy and other biological disciplines.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Bone Marrow

Growth Properties: Adherent

Morphology: Bipolar

Population Doubling: N/A

Seeding Density: 5,000 - 10,000 cells per cm²

Markers: N/A

Donor Age: 20 week gestation

Donor Gender: Female

Donor Ethnicity: Other

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 20% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C