Immortalized Human Bone Marrow Basophils

Immortalization Method: N/A

BioSafety Level: II

Species: Human

Source Organ: Bone Marrow

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: Grow cells in T25 ECM-coated flasks(G299) with the following conditions. The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0527.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C