Immortalized Human Astrocytes, fetal - SV40

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Fetal Brain

Growth Properties: Adherent

Morphology: Multipolar

Population Doubling: 49 - 59 hours

Seeding Density: 40,000-50,000 cells per cm². Recommended split ratio: 1:2

Markers: N/A

Donor Age: 18 weeks gestation

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 10ng/ml Human EGF (Z100135), 1% L-glutamine (G275), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C