Astrocytes are glial cells found in the brain and spinal cord that play a critical role in maintenance, support, and repair of nervous tissue. Astrocytes have also been implicated in various pathological processes. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. These cells can serve as valuable in vitro model for exploring the diversity of astrocytes functions.Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene.BioSafety Level: IISpecies: HumanSource Organ: BrainGrowth Properties: AdherentMorphology: MultipolarPopulation Doubling: 36 - 46 hoursSeeding Density: 20,000 - 40,000 cells per cm². Recommended split ratio: 1:3 - 1:4Markers: N/ADonor Age: 18 weeks gestationDonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 10ng/ml Human EGF (Z100135), 1% L-glutamine (G275), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C