Immortalized Human Adrenocortical Cells- hTERT

The human adrenal cortex plays an important role in regulating the endocrine system by secreting glucocorticoids, mineralcorticoids and adrenal androgens in response to environmental stimulation. The Immortalized Human Adrenocorticol Cells- hTERT is established from primary cells (isolated from a healthy kidney donor) using retrovirus carrying hTERT gene. These cells are useful in studies involving the biochemical and molecular mechanisms associated with adrenal steroidogenesis and adrenal physiology.

Immortalization Method: Serial passaging and transduction with recombinant retroviruses carrying hTERT gene

BioSafety Level: II

Species: Human

Source Organ: Adrenal gland

Growth Properties: Adherent

Morphology: Other

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 10% heat-inactivated horse serum, 0.1ng/ml recombinant Human FGF2 (Z101455) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1. Telomerase repeat amplification protocol (TRAP) assay was performed to measure telomerase activity in the immortalized cell line. 2) RT-PCR was used to quantify telomerase RNA component (TERC) expression in the immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C