Immortalized Hepatocyte Fusion-Like Cells

TERT-immortalized E12 MLPC were fused to human primary hepatocytes using a procedure adapted from a procedure used to create monoclonal antibodies using chemical fusion by polyethylene glycol

Immortalization Method: Transfected with the gene for TERT, cloned to pure populations by limited dilution culture and were expanded

BioSafety Level: II

Organism: Human (H. sapiens)

Species: Human

Source Organs: Liver

Organ Type: Liver

Growth Properties: Adherent

Morphology: Elongated then cuboidal

Seeding Density: 25,000-30,000 cells/cm²

Donor Gender: Male

Propagation: Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Expansion Medium Kit for Fusion Hepatocyte-like Cells (TM117), +37.0°C, 5% CO₂

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C

Reference: Collins, D. P., Hapke, J. H., Aravalli, R. N., & Steer, C. J. (2020). Development of immortalized human hepatocyte-like hybrid cells by fusion of multi-lineage progenitor cells with primary hepatocytes. PloS one, 15(6), e0234002. https://doi.org/10.1371/journal.pone.0234002