Description: Immortalized European Sea Bass Brain Cells (DLB-1) express glial cell markers and have epithelial morphology. Immortalized European Sea Bass Brain Cells (DLB-1) may be useful for studies of fish neurobiology and metal toxicity.Immortalization Method: Exposure to supernatant from SSN-1 cell line infected with snakehead retrovirusBioSafety Level: IISpecies: European Sea BassSource Organ: BrainGrowth Properties: AdherentMorphology: Epithelial-like to fibroblast-likePopulation Doubling: 18 - 28 hoursSeeding Density: N/AMarkers:gfap, coro1aDonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Leibovitz's L-15 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, L-glutamine (G275) to a final concentration of 2 mM, HEPES to a final concentration of 10 mM, and NaCl to a final concentration of 0.16%.
Temperature: 20 - 25°C.Quality Control: 1) Real time PCR; 2) Evaluation of metal cytotoxicityShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C