Immortalized Equine Lung Cells (extEqFL)

Immortalized Equine Lung Cells (extEqFL) are immortalized with the HPV16 E6/E7 gene through infection with non-replicating retrovirus carrying the PA317 LXSN HPV16 E6/E7 vector. These cells are a reliable tool for research in equine lung biology and can be used to assess the effects of equine related viruses. The extEqFL cells in vitro can be genetically manipulated for further research.

Immortalization Method: Infected with non-replicating retrovirus containing a supernatant from the PA317 LXSN HPV16E6E7

BioSafety Level: II

Species: Equine

Source Organ: Lung

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 72 hours

Seeding Density: 5x10⁴ cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 5%, 1% L-glutamine (G275), 600 µg/ml G418 (G271), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Selected cells with presence of 600 µg/ml G418, 2) PCR analysis for HPV16 E6/E7 presence.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C