Immortalized Equine Kidney Cells (extEqFK)

Description: Immortalized Equine Kidney Cells (extEqFK) are immortalized with the HPV16 E6/E7 gene through infection with non-replicating retrovirus carrying the PA317 LXSN HPV16 E6/E7 vector. In vitro, the cells can be genetically manipulated for further research. Thus are a reliable tool for research in equine kidney biology and can be used to assess the effects of equine related viruses.

Immortalization Method: Infected with non-replicating retrovirus containing a supernatant from the PA317 LXSN HPV16E6E7

BioSafety Level: II

Species: Equine

Source Organ: Kidney

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 62-72 hours

Seeding Density: 40, 000 cells/cm10² to 50, 000 cells/cm10²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 5%, 1% L-glutamine (G275), 600 µg/ml G418 (G271)**, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
**The addition of G418 should be added only after cells initially recover from thawing.

Quality Control: 1) Selected cells with presence of 600 µg/ml G418, 2) PCR analysis for HPV16 E6/E7 presence

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C