Species (Eptesicus fuscus) confirmed by sequencing of portion of Cytochrome B gene
Immortalization Method: Immortalized with Myotis Polyomavirus T antigen
BioSafety Level: II
Organism: Bat
Species: Bat
Source Organs: Kidney
Organ Type: Kidney
Growth Properties: Adherent
Morphology: Polyglonal
Population Doubling: 48 hours
Seeding Density: 13,000 - 15,000 cells/cm^2; Recommended split ratio 1:10
Donor Gender: Male
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 2 mM L-glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Banerjee, A., Rapin, N., Miller, M., Griebel, P., Zhou, Y., Munster, V., & Misra, V. (2016). Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen. Journal of virological methods, 237, 166–173. https://doi.org/10.1016/j.jviromet.2016.09.008