Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7

Mesenchymal stem cells are a multipotent population of cells capable of differentiating into a number of mesodermal lineages. Bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs) are potential cellular sources of therapeutic stem cells.Treatment using MSCs appears to be a helpful approach for structural restoration in regenerative medicine.

Immortalization Method: N/A

BioSafety Level: II

Species: Dog

Source Organ: Adipose Tissue

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: 50-60 hours

Seeding Density: N/A

Markers: Puromycin

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 0.2 ?g/ml puromycin (G264).
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1. Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line. 2. Puromycin selection

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C