Immortalization Method: Transfection of SV40 large T antigen plasmid with resistance to puromycin
BioSafety Level: II
Organism: Dog (Canine)
Species: Canine
Source Organs: Muscle
Organ Type: Vastus lateralis muscle
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 24 hours
Donor Age: 3 years old
Donor Gender: Female
Propagation: Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 20% FBS + 1X GlutaMAX + 2% chick embryo extract (Thermo Fisher) + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: López, S. M., Balog-Alvarez, C., Canessa, E. H., Hathout, Y., Brown, K. J., Vitha, S., Bettis, A. K., Boehler, J., Kornegay, J. N., & Nghiem, P. P. (2020). Creation and characterization of an immortalized canine myoblast cell line: Myok9. Mammalian genome : official journal of the International Mammalian Genome Society, 31(3-4), 95–109. https://doi.org/10.1007/s00335-020-09833-5