Immortalization Method: Immortalization with T SV40 (pSV3-neo vector)
BioSafety Level: II
Organism: Dog (Canine)
Species: Canine
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: Endothelial
Population Doubling: 72 hours
Seeding Density: 20,000-40,000 cells/cm
2Markers: eNOS, CD105 (by flow cytometry), CD146/MCAM, VEGFA, VEGFR1, VEGFR2 and CD271 (by qPCR)
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow C (TM100) + 2% heat-inactivated FBS + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂, G418 resistance (500 µg/ml)
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C