Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1)

Mesenchymal stromal cells are observed to have immunosuppressive effects on lymphocytes. The Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1) cells were generated from mononuclear cells derived from canine bone marrow. The cells were immortalized via transduction of a retrovirus containing the human papilloma virus E6/E7 genes. Phenotypic characterization of the Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1) cells via RT-PCR found that the cell line was weakly positive for CD10, CD29, CD106, moderately positive for CD73 and CD105, and strongly positive for CD90. The Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1) cells are applicable studies to investigate their immunosuppressive effects, especially in inflammatory and immune disease models.

Immortalization Method: Transduction with retrovirus containing the HPV E6/E7 genes

BioSafety Level: II

Species: Canine

Source Organ: Bone Marrow

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 15 - 20 hours

Seeding Density: 20,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, L-glutamine (G275) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Non-essential amino acid and sodium pyruvate may also be added.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C