Immortalization Method: Spontaneous immortalization
BioSafety Level: II
Organism: Cabbage Looper (T. ni)
Species: Cabbage Looper
Source Organs: Embryo
Organ Type: Embryo
Growth Properties: Adherent
Morphology: flat fibroblast-like
Seeding Density: Recommended split ratio is 1:8 or 1:12
Markers: Absence of alphanodavirus based on RT-PCR
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. TNMFH + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 27.0°C
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Hashimoto, Y., Zhang, S., Zhang, S., Chen, Y.-R., & Blissard, G. W. (2012). Correction: BTI-Tnao38, a new cell line derived from Trichoplusia ni, is permissive for AcMNPV infection and produces high levels of recombinant proteins. BMC Biotechnology, 12(1), 12. https://doi.org/10.1186/1472-6750-12-12