Immortalization Method: Spontaneous immortalization
BioSafety Level: II
Organism: Cow (Bovine)
Species: Bovine
Source Organs: Eye
Organ Type: Eye
Growth Properties: Adherent
Morphology: Oval to elongated
Markers: alpha-Smooth Muscle Actin, laminin and col IV (detected by ICC)
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow VI (TM016) + 10% FBS + 2 mM L-glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Mao, W., Liu, Y., Mody, A., Montecchi-Palmer, M., Wordinger, R. J., & Clark, A. F. (2012). Characterization of a spontaneously immortalized bovine trabecular meshwork cell line. Experimental eye research, 105, 53–59. https://doi.org/10.1016/j.exer.2012.10.007