Immortalization Method: Immortalization with T SV40 (pSV3-neo vector)
BioSafety Level: II
Organism: Cow (Bovine)
Species: Bovine
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: Endothelial
Population Doubling: 48 hours
Seeding Density: 20,000-40,000 cells/cm
2Markers: CD31-PECAM1, CD143-ACE, CD146-MCAM, VEGFA, VEGFR-1-Flt1, VEGFR-2-KDR-CD309, vWF, LYVE-1, CD117-KIT, CD133-PROM1, CXCR4-CD184
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow C (TM100) + 2% heat-inactivated FBS + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂, G418 resistance (80 µg/ml)
Quality Control: Mature endothelial cell, lymphatic endothelial cell, and progenitor endothelial cell markers detected by RT-qPCR
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Lagrée, A. C., Fasani, F., Rouxel, C., Pivet, M., Pourcelot, M., Fablet, A., Romey, A., Caignard, G., Vitour, D., Blaise-Boisseau, S., Kieda, C., Boulouis, H. J., Haddad, N., & Grillon, C. (2020). Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant
in vitro Models to Study Viral Infections. International journal of molecular sciences, 21(15), 5249. https://doi.org/10.3390/ijms21155249