Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T

The Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T were derived from primary embryonic cells from an 11-d-pregnant Bcl-x heterozygous mouse. The primary cells were immortalized by transfection with pCDNA3 plasmid encoding SV40 large T antigen. The immortalized cells display similar morphological characteristics with the parental cells. The Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T is useful in studies in discovering novel signaling pathways, investigating regulatory networks and immune responses particularly related to the Bcl-xL gene.

Immortalization Method: Transfection with pCDNA3 plasmid encoding SV40 large T antigen

BioSafety Level: II

Species: Mus musculus

Source Organ: Embryo

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 20 - 30 hours

Seeding Density: 20, 000 - 50, 000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C