Description: The Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T were derived from primary embryonic cells from an 11-d-pregnant Bcl-x heterozygous mouse. The primary cells were immortalized by transfection with pCDNA3 plasmid encoding SV40 large T antigen. The immortalized cells display similar morphological characteristics with the parental cells. The Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T is useful in studies in discovering novel signaling pathways, investigating regulatory networks and immune responses particularly related to the Bcl-xL gene.Immortalization Method: Transfection with pCDNA3 plasmid encoding SV40 large T antigenBioSafety Level: IISpecies: Mus musculusSource Organ: EmbryoGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: 20 - 30 hoursSeeding Density: 20, 000 - 50, 000 cells/cm10²Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C