Description: The Immortalized Baby Mouse Kidney Epithelial Cells (iBMK, Clone W2) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses epithelial-specific markers such as E-cadherin, cytokeratin 8 and 18. Together with Bax Knockdown iBMK (T3024), Bak Knockdown iBMK (T3025) and Bax/Bak Double Knockdown iBMK (T3026), these cell lines form the ideal system for studying mechanisms regulating apoptosis. In the same way, when paired with Atg5 Knockdown iBMK (T3027) and Atg7 Knockdown iBMK (T3028), they present an attractive model for investigating the relationship between autophagy and metabolism homeostasis. Immortalization Method: Serial passaging and electroporation transfection with linearized DNA of E1A and p53DDBioSafety Level: IISpecies: MouseSource Organ: KidneyGrowth Properties: AdherentMorphology: CuboidalPopulation Doubling: N/ASeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Markers: E-cadherin, CK8 and CK18Donor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression. Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C