Immortalized Adult Mouse Ventral Prostate Cells (INK4 -/- VP)

The Immortalized Adult Mouse Ventral Prostate Cells (INK4 -/- VP) were derived from a subline of the INK4a mouse, a transgenic knockout that lacks p16^INK4a and p19^ARF. Both p16^INK4aand p19^ARF are specific inhibitors of cyclin-dependent kinases Cdk4 and Cdk6 that regulate cell cycle progression. The cells were isolated from the ventral prostate (VP), which is lined by columnar epithelium that exhibits focal folding. The luminal secretory cells in the epithelium produce homogenous slightly basophilic secretion into the lumen of the ventral prostate. This cell line is a useful model for studying the prostate storma-epithelium signalling way, which plays an important role in prostate development and cancer progression.

Immortalization Method: Isolated from p16^INK4aand p19^ARF Knockout Transgenic Mice

BioSafety Level: II

Species: Mouse

Source Organ: Ventral Prostate

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Gentamycin (50ug/ml) + 1% ITS + Glutamine (1x) + 10^-8M DHT, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C