Immortalized Adult Mouse Coagulating Gland Cells (INK4 -/- CG)

The Immortalized Adult Mouse Coagulating Gland Cells (INK4 -/- CG)are derived from a subline of the INK4a mouse, a transgenic knockout that lacks p16^INK4aand p19^ARF. Both p16^INK4aand p19^ARF are specific inhibitors of cyclin-dependent kinases Cdk4 and Cdk6 that regulate cell cycle progression. The cells were isolated from the anterior prostate (AP), also known as the coagulating gland (CG), which has a unique cuboidal to columnar epithelium that has many mucosal folds projecting into the gland lumen. The lumen contains abundant eosinophilic secretions from the luminal secretory cells in the epithelium. This cell line is a useful model for studying the prostate stroma-epithelium signalling way, which plays an important role in prostate development and cancer progression.

Immortalization Method: Isolated from p16^INK4aand p19^ARF Knockout Transgenic Mice

BioSafety Level: II

Species: Mouse

Source Organ: Dorsolateral Prostate

Growth Properties: Adherent

Morphology: Fibroblast-like

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Gentamycin (50ug/ml) + 1% ITS + Glutamine (1x) + 10^-8M DHT, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C