UBG76V-GFP Stable HeLa Cell Line are derived from HeLa cells stably transfected to express ubiquitin mutant (UbG76V) and the GFP reporter. These cells are applicable for studies investigating the relationship between HPV E6/E7 proteins and ubiquitin-proteasome system. Quantity: 10⁶ cells/1.0 mlOrganism: HumanSource Organ: CervixBioSafety Level: IIGrowth Properties: AdherentMorphology: Epithelial-likePopulation Doubling: 18-26 hoursSeeding Density: 5,000 cells/cm2Markers: GFP, G418 resistance (0.8 mg/mL)Applications: For Research Use OnlyPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Preservation: 1. Freeze Medium: 90% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.Quality Control: 1) Western blot performed with anti-GFP antibodies (Cell Signaling Technology, #2555)Shipping Conditions: Dry IceStorage Conditions: -180°C