Stable HsCLN3 (LL/AA):RFP Expressing HEK293 Cell Line

HEK293 cells were transfected with pcDNA3.1 construct carrying HsCLN3 (LL/AA):RFP and were selected under the presence of G418 to create the Stable HsCLN3 (LL/AA):RFP Expressing HEK293 Cell Line Expressing HEK293 Cell Line to study these ion channels.

Quantity: 10⁶ cells/1.0 ml

Organism: Human (H. sapiens)

Source Organ: Kidney

BioSafety Level: II

Growth Properties: Adherent

Morphology: Epithelial-like

Population Doubling: 30-40 hours

Seeding Density: 30,000-50,000 cells/cm²

Markers: CLN3 LL/AA mutant, RFP

Applications: For Research Use Only

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% andPenicillin/Streptomycin Solution (G255) to a final concentration of 1%. 1.2 mg/mL G418 (G271) was used as the selection pressure for the cells. Change media every 2-3 days.Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.

Shipping Conditions: Dry Ice

Storage Conditions: -180°C