SCCF3 was derived from a 12-year old cat with a lingual squamous cell carcinoma. These cells were stably transfected with a plasmid containing a luciferase-YFP fusion construct.
Quantity: 10⁶ cells/1.0 ml
Organism: Cat (Feline)
Source Organ: Oral
BioSafety Level: II
Growth Properties: Adherent
Morphology: Epithelial
Markers: Luciferase, YFP, G418
Applications: For Research Use Only
Donor Age: 12 years old
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (>G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control: 1) Bioluminescence
Shipping Conditions: Dry Ice
Storage Conditions: -180°C