T6446 was developed from a laryngeal squamous cell carcinoma of a cat. The cells were stably transfected with a plasmid containing a luciferase-YFP fusion construct. SCCF1 cells can be used for experiments on genetic dysregulation in neoplastic keratinocytes of the feline oropharynx. These cells are a useful in vitro model for head and neck cancer.
Quantity: 1 million cells/1.0 ml
Organism: Cat (Feline)
Source Organ: Larynx
BioSafety Level: II
Growth Properties: Adherent
Morphology: Epithelial-like
Markers: G418, luciferase, YFP
Applications: For Research Use Only
Propagation: Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below. The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control: 1) Luminometer; 2) Flow cytometry
Shipping Conditions: Dry Ice
Storage Conditions: -180°C