THP-1 cells stably expressing RFP
Quantity: 10⁶ cells/1.0 ml
Organism: Human
Source Organ: Blood
BioSafety Level: II
Growth Properties: Suspension
Morphology: Round
Population Doubling: 30-35 hours
Seeding Density: 100,000-200,000 cells/ml
Markers: puromycin
Applications: For Research Use Only
Propagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 1mM of Sodium pyruvate (TM057), 2mM of L-Glutamine (G275), 10mM of HEPES, 1.2 µg/ml puromycin (G264), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Quality Control: 1) Puromycin drug selection RFP fluorescence evaluation
Shipping Conditions: Dry Ice
Storage Conditions: -180°C