PZ-TR Stable HepG2 Luciferase Reporter Cell Line is designed to assess the transcriptional activity of the human thyroid receptor (TR) through a luciferase reporter system. The presence of thyroid hormones, such as the triiodothyronine (T3), would induce luciferase activity and allows for function assessment. It is a valuable tool for the field of endocrine disruptor research as the cell line preserves the stoichiometric ratio between the human TR receptor and other transcriptional regulators. Quantity: 10⁶ cells/1.0 mlOrganism: HumanSource Organ: LiverBioSafety Level: IIGrowth Properties: AdherentMorphology: PolygonalSeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Applications: For Research Use OnlyOverexpression: Human thyroid receptor (TR) via transfection of transcriptionally active TR pGL4.14-TRE plasmid and selected by 500 μg/ml hygromycin B for clonal selectionPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 4 mM L-glutamine (G275), 1% non-essential amino acids, 1 mM sodium pyruvate (TM057), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. Recommended Split Ratio: 1:3 to 1:6Quality Control: 1) Resistance to 500 μg/ml hygromycin B for clonal selection; 2) Luciferase activity with 10 nM T3 after 48 hour incubation.Shipping Conditions: Dry IceStorage Conditions: -180°C