Muscarinic type 3 receptor (M3R) is the primary receptor subtype to promote fluid secretion in salivary acinar cells. Research has shown that anti-M3R autoantibodies (anti-M3R) are potential inhibitors of saliva secretion in Sjögren's syndrome (SjS), however there is currently no anti-M3R detection method established. M3R Stable Expressing HEK293 (M3R-GFP) Cell Line is applicable for studies on SjS. It can also be utilized for studies on chronic fatigue syndrome, and neurology or neural regulation-related studies as M3R is one of the important receptors for parasympathetic neurotransmitter, acetylcholine.Quantity: 10⁶ cells/1.0 mlOrganism: HumanSource Organ: KidneyBioSafety Level: IIGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: 36 hoursSeeding Density: 40,000-60,000 cells/cm2Applications: For Research Use OnlyPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999) to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and L-glutamine (G275) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.Quality Control: 1) Western blot; 2) Immunocytochemistry; 3) In-Cell Western assayShipping Conditions: Dry IceStorage Conditions: -180°C