Luciferase Stable U87MG/ΔEGFR Cell Line

Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. Luciferase stable was generated using abm's pLenti-III-UbC-Luciferase lentivirus on U87MG/ΔEGFR Cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (0.7 µg/ml)-resistant. Luciferase stable U87MG/ΔEGFR cells can be a very useful cell line for non-invasive visualization in in vitro experiments.

Quantity: 10⁶ cells/1.0 ml

Organism: Human

Source Organ: Glioblastoma; Brain

BioSafety Level: II

Growth Properties: Adherent

Population Doubling: 65 - 75 hours

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Applications: For Research Use Only

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Shipping Conditions: Dry Ice

Storage Conditions: -180°C