Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. Luciferase stable was generated using abm's pLenti-III-UbC-Luciferase lentivirus on U87MG/ΔEGFR Cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (0.7 µg/ml)-resistant. Luciferase stable U87MG/ΔEGFR cells can be a very useful cell line for non-invasive visualization in in vitro experiments.
Quantity: 1 million cells/1.0 ml
Organism: Human
Source Organ: Glioblastoma; Brain
BioSafety Level: II
Growth Properties: Adherent
Population Doubling: 65-75 hours
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications: For Research Use Only
Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Shipping Conditions: Dry Ice
Storage Conditions: -180°C